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KMID : 0378019600030120045
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New Medical Journal
1960 Volume.3 No. 12 p.45 ~ p.52
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Experimental Studies on the Influences of Prolonged Administration of a Large Dose of Cortisone Acetate to the Skeletal Muscle in Normal Rabbits
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Abstract
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During the course of the study with cortisone acetate, estradiol benzoate and testosterone propionate in normal rabbits, the group treated with cortisone showed progressive decrease of weight during the experiment while the remaining groups showed increase. The cortisone treated group showed also marked generalized atrophy of muscle, which attracted my interest. The present study is to investigate histopathologic changes of skeletal muscle due to the prolonged administration of a large dose of cortisone acetate in normal rabbits.
Twenty-eight rabbits,. 14 males and 14 females cif around 1, 000 grams were fed for one months with controled diet, then divided into three groups. Croup 1, fi males and 6 females, served as normal control; group 1, 5 males and 5 females, were given 10 mg/kg dose of cortisone acetate once every day for 45 days; and group 1, 3 males and 3 females, were given 10 mg/kg dose of cortisone acetate for 90 days. Four rabbits from group I were killed just after the beginning of experiment, another four at 45th day, and remaining four at 90th day. Rabbits in group 1 , were killed after the administration of cortisone for 45 E days, and rabbits in group M at 90th day. The specimen of muscle is taken from right psoas and left femoral area, and examined with H & E, van Gieson and PAS stain. Serum electrolytes (Na, K, Cl) were determined before, 45 days and 90 days after the beginning of experiment in surviving animals. The body weight was taken at every 10 day intervals in all surviving animals.
The result showed that a prolonged administration of a large dose of cortisone acetate in normal rabbits inhibited growth and produced degenerative lesions in skeletal muscle consisting of marked atrophy, parenchymatous degeneration, infiltration of macrophages and phagocytosis of degenerated muscle fibers, and proliferation of endomusial connective tissue. The lesion simulated that of potassium deficiency but the content-ration of serum potassium in normal control group and cortisone treated group did not show any notable difference. No alteration of nerve fiber present in examined sections is noted, and the lesion of muscle is similar to dystrophy rather than neurogenic atrophy. The exact mechanism that cortisone produces such wide spread lesion of skeletal. in normal rabbits is not clear and further investigation is desired.
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